Journal: Journal of Biomedicine and Biotechnology

Article Title: Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size

doi: 10.1155/2012/742829

Figure Lengend Snippet: Confocal microscopic observation of INHA cellular distribution in porcine oocytes isolated from small, medium, and large follicles before and after IVM. Porcine oocytes isolated from small, medium, and large follicles were stained before and after IVM with porcine INHA (before IVM; (a), (b), and (c) and after IVM; (d), (e), and (f)), (goat polyclonal anti-INHA, sc-22048). The treated oocytes were labeled for 40 min with FITC-conjugated anti-goat IgG Ab, which emits a green fluorescent signal after excitation at 488 nm at a 1 : 200 dilution in PBS. Bars are 50 μ m. L: large, M: medium, S: small follicles.

Article Snippet: Oocytes were incubated for 12 hours at 4°C with goat polyclonal anti-INHA antibody (Ab, sc-22048) or rabbit polyclonal anti-INHB (Ab, sc-50288) both from Santa Cruz Biotechnology (Santa Cruz, CA, USA), diluted 1 : 500 in PBS/1.5% BSA/0.1% Tween 20.

Techniques: Isolation, Staining, Labeling

Journal: Journal of Biomedicine and Biotechnology

Article Title: Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size

doi: 10.1155/2012/742829

Figure Lengend Snippet: Confocal microscopic observation of INHB cellular distribution in porcine oocytes isolated from small, medium, and large follicles before and after IVM. Porcine oocytes isolated from small, medium, and large follicles were stained before and after IVM with porcine INHB (before IVM; (a), (b), and (c) and after IVM; (d), (e), and (f)), (rabbit polyclonal anti-INHB, sc-50288). The treated oocytes were labeled for 40 min with TRITC (crystalline tetramethylrhodamine isothiocyanate), which emits a red fluorescent signal after excitation at 543 nm at a 1 : 200 dilution in PBS for INHB. Bars are 50 μ m. L: large, M: medium, S: small follicles.

Article Snippet: Oocytes were incubated for 12 hours at 4°C with goat polyclonal anti-INHA antibody (Ab, sc-22048) or rabbit polyclonal anti-INHB (Ab, sc-50288) both from Santa Cruz Biotechnology (Santa Cruz, CA, USA), diluted 1 : 500 in PBS/1.5% BSA/0.1% Tween 20.

Techniques: Isolation, Staining, Labeling

Journal: Molecular Medicine Reports

Article Title: Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure

doi: 10.3892/mmr.2016.5191

Figure Lengend Snippet: Primary antibodies, their source and dilutions.

Article Snippet: Rabbit anti-human/mouse inhibin β (sc-50288) , Santa Cruz Biotechnology, Inc. , 1:100.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure

doi: 10.3892/mmr.2016.5191

Figure Lengend Snippet: Immunofluorescence staining. Immunohistochemical staining indicated that ovarian granulosa-like cells derived from induced pluripotent stem cells strongly expressed ovarian granulosa cells markers [anti-Müllerian hormone (AMH), inhibin α, inhibin β and follicle-stimulating hormone receptor (FSHR)], but not stem cell markers [octamer-binding transcription factor 4 (Oct4), SRY (sex-determining region Y)-box 2 (Sox2), Nanog and stage-specific embryonic antigen-4 (SSEA4) 12 days post-induction. Each sample was stained for two markers with the color of the fluorescence indicated by the color of the word. Original magnification, ×200. Cy3, cyanine 3; DAPI, 4′,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate.

Article Snippet: Rabbit anti-human/mouse inhibin β (sc-50288) , Santa Cruz Biotechnology, Inc. , 1:100.

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Derivative Assay, Binding Assay, Fluorescence